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2025, Vol: 3, Issue: 2
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Original Research 


Identification and Functional Analysis of miR-204-3p in Oral Squamous Cell Carcinoma: Insights into Target Gene and Expression Patterns in Indian Population- A Pilot Study

Ishwarya S, Ashikha Shirin Usman Pp, Ameya Kp, Dhanraj M Ganapathy, Durairaj Sekar.


Abstract
Introduction
Oral Squamous Cell Carcinoma (OSCC) is a significant global health issue, originating from squamous cells in the oral cavity. Tumor necrosis factor-alpha (TNF-α), a pro-inflammatory cytokine, plays a crucial role in promoting cell proliferation, metastasis, and invasion in OSCC. This study investigates the role of miR-204-3p in regulating TNF-α, exploring its potential as a therapeutic target and its impact on cancer progression.
Aim
The primary aim of this study is to explore the regulatory relationship between miR-204-3p and TNF-α in OSCC, focusing on miR-204-3p's potential role as a tumor suppressor and its implications for targeted therapies in OSCC.
Methods
Human genome sequences were obtained from NCBI, and OSCC-specific sequences were filtered to create a nucleotide database. miRNA reference sequences were sourced from miRBase, and miRNA targets were predicted using TargetScan based on conserved binding sites. The structural stability of precursor-miRNAs was validated using RNAfold. OSCC tissue samples were ethically collected, and total RNA was isolated using TRIzol. Gene expression levels of TNF-α and miR-204-3p were analyzed using qRT-PCR, with statistical analysis performed using SPSS.
Results
The study identified hsa-miR-204-3p as a regulator of TNF-α, characterized by a stable stem-loop structure. qRT-PCR analysis revealed significant downregulation of miR-204-3p and upregulation of TNF-α in OSCC tissues compared to normal tissues.
Conclusion
hsa-miR-204-3p may act as a tumor suppressor in OSCC by targeting TNF-α, suggesting its potential as a therapeutic target for OSCC treatment.

Key words: Micro-RNA, Oral Squamous Cell Carcinoma, Therapeutic target, TNF-α


 
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How to Cite this Article
Pubmed Style

S I, Pp ASU, Kp A, Ganapathy DM, Sekar D. Identification and Functional Analysis of miR-204-3p in Oral Squamous Cell Carcinoma: Insights into Target Gene and Expression Patterns in Indian Population- A Pilot Study. A-JMRHS . 2025; 3(1): 8-14. doi:10.5455/AJMRHS.20250503

Web Style

S I, Pp ASU, Kp A, Ganapathy DM, Sekar D. Identification and Functional Analysis of miR-204-3p in Oral Squamous Cell Carcinoma: Insights into Target Gene and Expression Patterns in Indian Population- A Pilot Study. https://www.ajmrhs.com/?mno=218555 [Access: August 15, 2025]. doi:10.5455/AJMRHS.20250503

AMA (American Medical Association) Style

S I, Pp ASU, Kp A, Ganapathy DM, Sekar D. Identification and Functional Analysis of miR-204-3p in Oral Squamous Cell Carcinoma: Insights into Target Gene and Expression Patterns in Indian Population- A Pilot Study. A-JMRHS . 2025; 3(1): 8-14. doi:10.5455/AJMRHS.20250503


Vancouver/ICMJE Style

S I, Pp ASU, Kp A, Ganapathy DM, Sekar D. Identification and Functional Analysis of miR-204-3p in Oral Squamous Cell Carcinoma: Insights into Target Gene and Expression Patterns in Indian Population- A Pilot Study. A-JMRHS . (2025), [cited August 15, 2025]; 3(1): 8-14. doi:10.5455/AJMRHS.20250503


Harvard Style

S, I., Pp, . A. S. U., Kp, . A., Ganapathy, . D. M. & Sekar, . D. (2025) Identification and Functional Analysis of miR-204-3p in Oral Squamous Cell Carcinoma: Insights into Target Gene and Expression Patterns in Indian Population- A Pilot Study. A-JMRHS , 3 (1), 8-14. doi:10.5455/AJMRHS.20250503


Turabian Style

S, Ishwarya, Ashikha Shirin Usman Pp, Ameya Kp, Dhanraj M Ganapathy, and Durairaj Sekar. 2025. Identification and Functional Analysis of miR-204-3p in Oral Squamous Cell Carcinoma: Insights into Target Gene and Expression Patterns in Indian Population- A Pilot Study. Asian Journal of Medical Research & Health Sciences (A-JMRHS), 3 (1), 8-14. doi:10.5455/AJMRHS.20250503


Chicago Style

S, Ishwarya, Ashikha Shirin Usman Pp, Ameya Kp, Dhanraj M Ganapathy, and Durairaj Sekar. "Identification and Functional Analysis of miR-204-3p in Oral Squamous Cell Carcinoma: Insights into Target Gene and Expression Patterns in Indian Population- A Pilot Study." Asian Journal of Medical Research & Health Sciences (A-JMRHS) 3 (2025), 8-14. doi:10.5455/AJMRHS.20250503


MLA (The Modern Language Association) Style

S, Ishwarya, Ashikha Shirin Usman Pp, Ameya Kp, Dhanraj M Ganapathy, and Durairaj Sekar. "Identification and Functional Analysis of miR-204-3p in Oral Squamous Cell Carcinoma: Insights into Target Gene and Expression Patterns in Indian Population- A Pilot Study." Asian Journal of Medical Research & Health Sciences (A-JMRHS) 3.1 (2025), 8-14. Print. doi:10.5455/AJMRHS.20250503


APA (American Psychological Association) Style

S, I., Pp, . A. S. U., Kp, . A., Ganapathy, . D. M. & Sekar, . D. (2025) Identification and Functional Analysis of miR-204-3p in Oral Squamous Cell Carcinoma: Insights into Target Gene and Expression Patterns in Indian Population- A Pilot Study. Asian Journal of Medical Research & Health Sciences (A-JMRHS), 3 (1), 8-14. doi:10.5455/AJMRHS.20250503




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