THE STUDY OF VITAMIN D RECEPTOR GENE POLYMORPHISM, PTH AND CALCIUM LEVELS IN 25-HYDROXYVITAMIN D DEFICIENCY ARE CAUSE TO HYPERTENSION

Abstract

Hypertension is a multifactorial and complex disease with high rate of incidence and associated co-morbidities. Previous studies do not provide unanimous results for the risk of hypertension and association with Fok I genotype frequency, serum vitamin D levels along with Ca+ levels. Hence, this study was undertaken to determine the status of Fok I vitamin D receptor (VDR) gene polymorphism along with vitamin D, Ca+ levels and blood pressure in patients with hypertension. Two hundred (100 controls and 100 cases of hypertension) participants from general Indian population were enrolled in this study. Peripheral blood samples were collected for genotyping Fok I-VDR gene polymorphism using PCR–RFLP method whereas 25-OH vitamin D levels in serum were quantified using high performance liquid chromatography (HPLC). Significantly reduced 25-OH vitamin D, Ca+ levels were observed in patients with hypertension (19.31 ± 7.42 vs 48.20 ± 14.10) com- pared to control subjects (p = 0.0001). Homozygous recessive genotype ‘ff’ frequency was increased by 8.06 fold (CI: 3.14–16.42, p = 0.0001) in patients with hypertension compared to dominant ‘FF’ genotype frequency. In conclusion, recessive ‘ff’ genotype frequency correlates with reduced serum vitamin D, Ca+ levels and results in significantly increased systolic and diastolic blood pressures leading to predisposition of hypertension.